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HSL Manual Haematology (Immunophenotyping and Immune Monitoring)

HSL’s Immunophenotyping and Immune Monitoring laboratory is equipped with a suite of state-of-the art 10-colour 3-laser flow cytometers. We use a sophisticated automated lysing process that allows 40 markers of differentiation to be interrogated, diagnosing malignancies with a rapid turnaround time.

The Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) coordinates, manages and guides laboratory scientists and clinicians towards a fully comprehensive, streamlined and rapid diagnostic and monitoring service to the patient. Our repertoire includes HIV Immune (CD4:CD8) and CD19 monitoring.

The SIHMDS diagnostic algorithm, drafted and updated by haematology oncology consultants, includes the disciplines of immunophenotyping, cytogenetics, histology and molecular testing of acute leukaemia (myeloid, T-cell, B-cell), chronic leukaemia (T-CLL, B-CLL, B-PLL) and lymphoma (MCL, FL, HCL and other) on bodily fluids.

At the dedicated SIHMDS reception, staff carry out pre- analytical work such as data entry, processing slides, lymphocyte subsets and calibrating analysers.

Morphological screenings, where level of work-up is discussed, are attended by scientists and medical staff. Samples are then dispatched to the correct disciplines, and data are entered onto an integrated reporting system.

Customised panels are used, pre-prepared by the manufacturer with full QC and titre information. Panel contents are selected by scientists and clinical consultants from WHO’s ‘Classification: Tumours of Haematopoietic and Lymphoid Tissues’.

All laboratory staff process malignancy samples, and fully trained BMSs interpret and report onto LIS; authorisation includes consultants and laboratory staff. An integrated report for all disciplines with a joint conclusion is made and reported by consultant staff.

We hold the IBMS pre- and post- registration training approval for completion of the IBMS Registration Portfolio and Specialist Diplomas. HSL Manual Haematology (Immunophenotyping and Immune Monitoring) is a UKAS Accredited Medical Laboratory No. 8169.

General enquiries

Out of hours service

Acute Leukaemia Screen + MRD

An out-of-hours service in place covering Saturday and Sunday and all Bank Holidays, 9.00am–3.00pm. This is for undiagnosed new acute leukaemias which have to be treated BEFORE next working day. All call-out have to be authorised by one of the consultants named above.

Bone Marrow Aspirate (BMA)

There is no out of hours service for BM aspirate reporting unless it is part of an undiagnosed new acute leukaemia screen where a consultant has authorised on-call work to be carried out.



See the main Tests section for general information on ordering tests, and specimen collection, packaging and transport.

  • All citrate samples sent by post or with an overnight delay must be double spun and sent frozen.
  • Samples must be labelled correctly with three points of ID.
  • Samples must be left in correct courier pick-up point.

Please send samples to:

Health Services Laboratories
Manual Haematology
(Immunophenotyping and Immune Monitoring) Halo Level 2
The Halo Building
1 Mabledon Place
London WC1H 9AX


Request procedures

See the main Tests section for general information on request procedures.